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Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins
- 1.0359775 - ÚŽFG 2012 RIV GB eng J - Journal Article
Procházka, Radek - Petlach, Michal - Nagyová, Eva - Němcová, Lucie
Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation and acquisition of developmental competence in vitro: comparison with gonadotropins.
Reproduction. Roč. 141, č. 4 (2011), s. 425-435. ISSN 1470-1626. E-ISSN 1741-7899
R&D Projects: GA ČR GA523/08/0111; GA MZe QI101A166
Institutional research plan: CEZ:AV0Z50450515
Keywords : activatd protein kinase * EGF like factor * meiotic resumption
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.090, year: 2011
The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation and acquisition of developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but notbetacellulin (BTC) in the cultured COCs. Both FSH and the EGF-like peptides significantly stimulated expression of expansion related genes (PTGS2, TNFAIP6 and HAS2) in the COCs and stimulated maturation of oocytes and expansion of cumulus cells. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. Significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or PMSG/hCG.
Permanent Link: http://hdl.handle.net/11104/0197489
Number of the records: 1