Prophase I arrest and progression to metaphase I in mouse oocytes: comparison of resumption of meiosis and recovery from G2-arrest in somatic cells

0359477 - ÚŽFG 2012 RIV GB eng J - Journal Article
Šolc, Petr - Schultz, R. M. - Motlík, Jan
Prophase I arrest and progression to metaphase I in mouse oocytes: comparison of resumption of meiosis and recovery from G2-arrest in somatic cells.
Molecular Human Reproduction. Roč. 16, č. 9 (2010), s. 654-664. ISSN 1360-9947
R&D Projects: GA ČR(CZ) GC301/09/J036; GA MŠMT ME08030
Grant - others:NIH(US) HD22681
Institutional research plan: CEZ:AV0Z50450515
Keywords : resumption of meiosis * prophase I arrest * oocyte
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.506, year: 2010

Mammalian oocytes are arrested at prophase I until puberty when luteinizing hormone (LH) induces resumption of meiosis of follicle-enclosed oocytes. Resumption of meiosis is tightly coupled with regulating cyclin-dependent kinase 1 (CDK1) activity. Prophase I arrest depends on inhibitory phosphorylation of CDK1 and anaphase-promoting complex-(APC-CDH1)-mediated regulation of cyclin B levels. Prophase I arrest is maintained by endogenously produced cyclic adenosine monophosphate (cAMP), which activates protein kinase A (PKA) that in turn phosphorylates (and activates) the nuclear kinase WEE2. In addition, PKA-mediated phosphorylation of the phosphatase CDC25B results in its cytoplasmic retention. The combined effect maintains low levels of CDK1 activity that are not sufficient to initiate resumption of meiosis.
Permanent Link: http://hdl.handle.net/11104/0197261