DEAD-box RNA helicase is dispensable for mitochondrial translation in Trypanosoma brucei

0359433 - BC 2012 RIV US eng J - Journal Article
Richterová, Lenka - Vávrová, Zuzana - Lukeš, Julius
DEAD-box RNA helicase is dispensable for mitochondrial translation in Trypanosoma brucei.
Experimental Parasitology. Roč. 127, č. 1 (2011), 300-303. ISSN 0014-4894. E-ISSN 1090-2449
R&D Projects: GA ČR GA204/09/1667; GA MŠMT LC07032; GA MŠMT 2B06129
Institutional research plan: CEZ:AV0Z60220518
Keywords : Trypanosoma * Mitochondrial translation * RNA helicase * Cytochrome c oxidase * Mitochondrion
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.122, year: 2011

DEAD-box RNA helicase, a putative subunit of the mitochondrial ribosome of Trypanosoma brucei, has been down-regulated in the procyclic and bloodstream stage by RNA interference. Although ablation of the transcript leads to a week growth phenotype in the procyclic cells, the protein does not seem to be essential for mitochondrial translation under standard cultivation conditions, as shown by an assay that allows visualization of the de novo synthesized proteins. Furthermore, we show that synthesis of cytochrome c oxidase subunit I and cytochrome b does not occur in the mitochondrion of the bloodstream stage.
Permanent Link: http://hdl.handle.net/11104/0197226