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Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for selective isolation of bacterial DNA
- 1.0359412 - ÚMCH 2012 RIV GB eng J - Journal Article
Horák, Daniel - Španová, A. - Tvrdíková, J. - Rittich, B.
Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microspheres for selective isolation of bacterial DNA.
European Polymer Journal. Roč. 47, č. 5 (2011), s. 1090-1096. ISSN 0014-3057. E-ISSN 1873-1945
R&D Projects: GA MŠMT 2B06053; GA ČR GA203/09/1242; GA ČR GA203/09/0857; GA MŠMT 7E09109; GA ČR GAP503/10/0664
Institutional research plan: CEZ:AV0Z40500505
Keywords : glycidyl methacrylate * 2-hydroxyethyl methacrylate * magnetic
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.739, year: 2011
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres which were functionalized with streptavidin. DNA sensor was constructed for magnetic separation of DNA from real samples.
Permanent Link: http://hdl.handle.net/11104/0006440
Number of the records: 1