Borrelia carolinensis sp. nov., a novel species of the Borrelia burgclorferi sensu lato complex isolated from rodents and a tick from the south-eastern USA

0359403 - BC 2012 RIV GB eng J - Journal Article
Rudenko, Natalia - Golovchenko, Maryna - Grubhoffer, Libor - Oliver, J. H., Jr.
Borrelia carolinensis sp. nov., a novel species of the Borrelia burgclorferi sensu lato complex isolated from rodents and a tick from the south-eastern USA.
International Journal of Systematic and Evolutionary Microbiology. Roč. 61, č. 2 (2011), 381-383. ISSN 1466-5026. E-ISSN 1466-5034
R&D Projects: GA MŠMT(CZ) LC06009; GA ČR GA206/09/1782
Institutional research plan: CEZ:AV0Z60220518
Keywords : MULTILOCUS SEQUENCE-ANALYSIS * FRAGMENT-LENGTH-POLYMORPHISM * LYME-DISEASE * RIBOSOMAL-RNA * Borrelia carolinensis sp. nov. * phylogeny
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.268, year: 2011

A group of 16 isolates with genotypic characteristics different from those of known species of the Borrelia burgdorferi sensu lato complex were cultured from ear biopsies of the rodents Peromyscus gossypinus and Neotoma floridana trapped at five localities in South Carolina, USA, and from the tick Ixodes minor feeding on N. floridana. Multilocus sequence analysis of members of the novel species, involving the 16S rRNA gene, the 5S-23S (rrf-rrl) intergenic spacer region and the flagellin, ospA and p66 genes, was conducted and published previously and was used to clarify the taxonomic status of the novel group of B. burgdorferi sensu lato isolates. Phylogenetic analysis based on concatenated sequences of the five analysed genomic loci showed that the 16 isolates clustered together but separately from other species in the B. burgdorferi sensu lato complex. The analysed group therefore represents a novel species, formally described here as Borrelia carolinensis sp. nov.
Permanent Link: http://hdl.handle.net/11104/0197200