Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry

0359308 - ÚEB 2011 RIV NL eng J - Journal Article
Tarkowski, Petr - Václavíková, Kateřina - Novák, Ondřej - Pertry, I. - Hanuš, Jan - Whenham, R. - Vereecke, D. - Šebela, M. - Strnad, Miroslav
Analysis of 2-methylthio-derivatives of isoprenoid cytokinins by liquid chromatography-tandem mass spectrometry.
Analytica Chimica Acta. Roč. 680, 1-2 (2010), s. 86-91. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA ČR GA301/08/1649
Institutional research plan: CEZ:AV0Z50380511
Keywords : Cytokinins * Rhodococcus fascians * High-performance liquid chromatography
Subject RIV: CE - Biochemistry
Impact factor: 4.310, year: 2010

A sensitive and reliable high-performance liquid chromatographic method with tandem mass spectrometric detection has been developed and used for the determination of 2-methylthio-cytokinin derivatives produced by the phytopathogenic actinomycete Rhodococcus fascians. The cultivation medium containing secreted cytokinins was concentrated and subjected to a solid-phase extraction (C18 and ion-exchange). The purified samples were further separated and analyzed by HPLC-ESI-MS/MS. This allowed to achieve chromatographic resolution of six highly hydrophobic cytokinin species including 2-methylthio-isopentenyladenine, 2-methylthio-isopentenyladenosine, 2-methylthio-trans-zeatin and 2-methylthio-trans-zeatin riboside and their cis-isomers. Quantification was performed by a standard isotope dilution method using a multiple-reaction monitoring (MRM) mode.
Permanent Link: http://hdl.handle.net/11104/0197112