A subset of cytokinin two-component signaling system plays a role in cold temperature stress response in Arabidopsis

0359299 - ÚEB 2011 RIV US eng J - Journal Article
Jeon, J. - Kim, N. Y. - Kim, S. - Kang, N. Y. - Novák, Ondřej - Ku, S. J. - Cho, D. J. - Lee, D. J. - Lee, E. J. - Strnad, Miroslav - Kim, J.
A subset of cytokinin two-component signaling system plays a role in cold temperature stress response in Arabidopsis.
Journal of Biological Chemistry. Roč. 285, č. 30 (2010), s. 23369-23384. ISSN 0021-9258. E-ISSN 1083-351X
R&D Projects: GA ČR GA301/08/1649
Institutional research plan: CEZ:AV0Z50380511
Keywords : Histidine kinase * Gene-expression * Negative regulator
Subject RIV: EF - Botanics
Impact factor: 5.328, year: 2010

A multistep two-component signaling system is established as a key element of cytokinin (CK) signaling in Arabidopsis. AHK2 and AHK3 were found to be primarily involved in mediating cold to express A-type ARRs despite CK deficiency. Cold neither significantly induced AHK2 and AHK3 expression nor altered the CK contents of wild type within the 4 h during which the A-type ARRgenes exhibited peak expression in response to cold, indicating that cold might induce ARR expression via the AHK2 and AHK3 proteins. The overexpression of the cold-inducible ARR7 in Arabidopsis resulted in a hypersensitivity response to freezing temperatures under cold acclimated conditions. The ahk2 ahk3 and arr7 mutants showed hypersensitive response to abscisic acid (ABA) for germination. These results suggest that AHK2 and AHK3 and the cold-inducible A-type ARRs play a negative regulatory role in cold stress signaling via inhibition of ABA response, occurring independently of the cold acclimation pathway.
Permanent Link: http://hdl.handle.net/11104/0197106