Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells

0359295 - ÚEB 2011 RIV US eng J - Journal Article
Koledová, Z. - Rašková-Kafková, L. - Calábková, L. - Kryštof, Vladimír - Doležel, P. - Divoký, V.
Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells.
Stem Cells and Development. Roč. 19, č. 2 (2010), s. 181-193. ISSN 1547-3287. E-ISSN 1557-8534
R&D Projects: GA ČR GA301/08/1649
Institutional research plan: CEZ:AV0Z50380511
Keywords : embryonic stem cells * cell cycle * G1 phase
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.791, year: 2010

Embryonic stem cells (ESCs) proliferate rapidly and have a unique cell-cycle structure with a very short G1 phase. Previous reports suggested that the rapid G1 phase progression of ESCs might be underpinned by high and precocious Cdk2 activity and that Cdk2 activity might be crucial for both cell-cycle regulation and cell-fate decisions in human ESCs. However, the actual role of Cdk2 in cell-cycle progression of mouse ESCs (mESCs) has not been elucidated. In this study, we investigated the effects of down-regulation of Cdk2 activity by olomoucine II in 2 mESC lines. Olomoucine II treatment signifi cantly increased the G1 phase cell numbers, decreased the S phase cell numbers, and inhibited DNA replication in mESCs. In nocodazole-synchronized mESCs, we show that specifi c down-regulation of Cdk2 activity prolongs G1 phase progression. In addition, down-regulation of Cdk2 activity in mESCs established a somatic cell-like cell cycle and induced expression of differentiation markers.
Permanent Link: http://hdl.handle.net/11104/0198840