Endogenous cytokinins in Cocos nucifera L. in vitro cultures obtained from plumular explants

0359284 - ÚEB 2011 RIV DE eng J - Journal Article
Sáenz, L. - Azpeitia, A. - Oropeza, C. - Jones, L. - Fuchsová, Květoslava - Spíchal, Lukáš - Strnad, Miroslav
Endogenous cytokinins in Cocos nucifera L. in vitro cultures obtained from plumular explants.
Plant Cell Reports. Roč. 29, č. 11 (2010), s. 1227-1234. ISSN 0721-7714. E-ISSN 1432-203X
R&D Projects: GA ČR GA301/08/1649; GA AV ČR IBS5038351
Institutional research plan: CEZ:AV0Z50380511
Keywords : Coconut palm * Somatic embryogenesis * Endogenous cytokinins
Subject RIV: EF - Botanics
Impact factor: 2.279, year: 2010

Embryogenic calli and non-embryogenic calli can be formed from the initial calli. Fourteen cytokinins were analysed in the three types of calli and all the cytokinins were found in each type. The most abundant cytokinins in each type of callus were isopentenyladenine-9-glucoside, zeatin-9-glucoside, zeatin riboside, isopentenyladenine riboside, dihydrozeatin and dihydrozeatin riboside in decreasing order. Total cytokinin content was found to be lower in embryogenic calli compared to non-embryogenic calli or initial calli. When explants were cultured in media containing exogenously added cytokinins, the formation of embryogenic calli in the explants was reduced. When 8-azaadenine (an anticytokinin) was added the formation of embryogenic calli and static embryos was increased. These results suggest that the difference in somatic embryo formation capacity observed between embryogenic calli and non-embryogenic calli is related to their endogenous cytokinin contents.
Permanent Link: http://hdl.handle.net/11104/0197095