PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca

0359234 - ÚŽFG 2012 RIV GB eng J - Journal Article
Lajbner, Zdeněk - Kotlík, Petr
PCR-RFLP assays to distinguish the Western and Eastern phylogroups in wild and cultured tench Tinca tinca.
Molecular Ecology Resources. Roč. 11, č. 2 (2011), s. 374-377. ISSN 1755-098X. E-ISSN 1755-0998
R&D Projects: GA MŠMT LC06073
Institutional research plan: CEZ:AV0Z50450515
Keywords : EPIC * exon-primed intron-crossing marker * mtDNA
Subject RIV: EG - Zoology
Impact factor: 3.062, year: 2011

The tench Tinca tinca is a valued table fish which is being transplanted between watersheds without concern for genetic similarity to wild populations, and efficient phylogeographic markers were therefore urgently needed to rapidly distinguish genetically distinct geographic populations and to assess their contribution to the hatchery breeds and to the stocked wild populations. The new method developed in this study relies on PCR-RFLP assays of two independent nuclear-encoded markers and of one mitochondrial DNA marker, and allows the rapid identification of the Western and Eastern phylogroup and also of three geographical mtDNA clades within the Eastern phylogroup. The method will be useful for monitoring the introduction and human-mediated spread of the phylogroups in wild populations, for characterisation of cultured strains and in breeding experiments.
Permanent Link: http://hdl.handle.net/11104/0197058