Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes

0359225 - BC 2012 RIV NL eng J - Journal Article
Kváč, Martin - Hromadová, N. - Květoňová, Dana - Rost, M. - Sak, Bohumil
Molecular characterization of Cryptosporidium spp. in pre-weaned dairy calves in the Czech Republic: Absence of C. ryanae and management-associated distribution of C. andersoni, C. bovis and C. parvum subtypes.
Veterinary Parasitology. Roč. 177, 3/4 (2011), s. 378-382. ISSN 0304-4017. E-ISSN 1873-2550
Institutional research plan: CEZ:AV0Z60220518
Keywords : Calves * Cryptosporidium andersoni * C. bovis * C. parvum * GP60 * SSU
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 2.579, year: 2011

A total of 750 faecal samples of dairy calves kept in various housing systems were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method.DNAwas extracted from Cryptosporidium positive samples and from 150 randomly selected microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA gene that was subsequently digested by SspI, VspI and MboII restriction enzymes. In addition, the C. parvum samples were subsequently analyzed at the GP60 gene to determine the distribution of zoonotic subtypes. Sequence analyses and RFLP identified C. parvum in 137, C. andersoni in 21 and C. bovis in 3 samples. Neither mixed infections nor C. ryanae was detected. IIaA15G2R1 and IIaA16G1R1 were detected as major subtypes. 3 of the 5 detected subtypes were previously associated with human cryptosporidiosis, and moreover, the IIaA15G1R1 subtype, previously reported in humans only, was detected in calves for the first time.
Permanent Link: http://hdl.handle.net/11104/0197052