Diverse retrotransposon families and an AT-rich satellite DNA revealed in giant genomes of Fritillaria lilies

0359134 - BC 2011 RIV GB eng J - Journal Article
Ambrožová, K. - Mandáková, T. - Bureš, P. - Neumann, Pavel - Leitch, I. J. - Koblížková, Andrea - Macas, Jiří - Lysák, M.
Diverse retrotransposon families and an AT-rich satellite DNA revealed in giant genomes of Fritillaria lilies.
Annals of Botany. Roč. 107, č. 2 (2011), s. 255-268. ISSN 0305-7364. E-ISSN 1095-8290
R&D Projects: GA ČR GA521/07/0284; GA MŠMT(CZ) LC06004
Institutional research plan: CEZ:AV0Z50510513
Keywords : Fritillaria * Liliaceae * repetitive DNA
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.030, year: 2011

Background and Aims The genus Fritillaria (Liliaceae) comprises species with extremely large genomes (1C = 30 000-127 000 Mb) and a bicontinental distribution. Most North American species (subgenus Liliorhiza) differ from Eurasian Fritillaria species by their distinct phylogenetic position and increased amounts of heterochromatin. This study examined the contribution of major repetitive elements to the genome obesity found in Fritillaria and identified repeats contributing to the heterochromatin arrays in Liliorhiza species. Methods Two Fritillaria species of similar genome size were selected for detailed analysis, one from each phylogeographical clade: F. affinis (1C = 45.6 pg, North America) and F. imperialis (1C = 43.0 pg, Eurasia). Fosmid libraries were constructed from their genomic DNAs and used for identification, sequence characterization, quantification and chromosome localization of clones containing highly repeated sequences.
Permanent Link: http://hdl.handle.net/11104/0196974