Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine

0359122 - ÚMCH 2012 RIV NL eng J - Journal Article
Šlouf, Miroslav - Pavlova, Ewa - Bhardwaj, Monika - Pleštil, Josef - Onderková, H. - Philimonenko, Anatoly - Hozák, Pavel
Preparation of stable Pd nanoparticles with tunable size for multiple immunolabeling in biomedicine.
Materials Letters. Roč. 65, č. 8 (2011), s. 1197-1200. ISSN 0167-577X. E-ISSN 1873-4979
R&D Projects: GA AV ČR KAN200520704; GA ČR GAP205/10/0348
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50520514
Keywords : palladium nanoparticles * nanocrystalline materials * immunolabeling
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.307, year: 2011

Stable Pd nanoparticles (PdNP) with a tunable size (3-15 nm) were synthesized by controlled chemical reduction of PdCl2 with sodium citrate in water. The morphology of PdNP was characterized by transmission electron microscopy, while their stability in solution was verified by quasi-elastic light scattering and small-angle X-ray scattering. Intensive stirring of reacting mixture played a vital role in achieving reproducible particle sizes. Controlled changes of pH and initial concentrations were employed in fine-tuning particle size distributions. Finally, 10 nm PdNP were conjugated with goat anti-mouse IgG antibody as proved by electrophoresis (SOS-PAGE) and used for ultrastructural immunolabeling, which confirmed suitability of PdNP for multiple immunolabeling in biomedicine.
Permanent Link: http://hdl.handle.net/11104/0006427