Glycerol-3-phosphate Dehydrogenase Expression and Oxygen Consumption in Liver Mitochondria of Female and Male Rats with Chronic Alteration of Thyroid Status

0359114 - FGÚ 2012 RIV DE eng J - Journal Article
Rauchová, Hana - Mráček, Tomáš - Novák, Petr - Vokurková, Martina - Soukup, Tomáš
Glycerol-3-phosphate Dehydrogenase Expression and Oxygen Consumption in Liver Mitochondria of Female and Male Rats with Chronic Alteration of Thyroid Status.
Hormone and Metabolic Research. Roč. 43, č. 1 (2011), s. 43-47. ISSN 0018-5043. E-ISSN 1439-4286
R&D Projects: GA ČR GA303/09/0570; GA ČR(CZ) GA304/08/0256; GA ČR GPP303/10/P227; GA MŠMT(CZ) 1M0510
Institutional research plan: CEZ:AV0Z50110509
Keywords : chronic administration * triiodothyronine * methimazole * sex difference * idebenone
Subject RIV: ED - Physiology
Impact factor: 2.188, year: 2011

In our chronic experiments the activity and protein amount of glycerol-3-phosphate dehydrogenase (GPDH) in mitochondria isolated from the liver of adult male and female inbred Lewis strain euthyroid (EU), hyperthyroid (TH) and hypothyroid (HY) rats were analyzed by biochemical and Western blot methods. The TH status led to a significant increase and HY status to a significant decrease of enzyme activity and protein amount in both male and female animals. These changes were, however, more pronounced in females. Measurements of serum thyroid hormone levels and analysis of anatomical parameters confirmed that our procedures inducting the TH and HY states are efficient and reliable and that determination of GPDH can serve as an additional criterion for the evaluation of the thyroid hormone status
Permanent Link: http://hdl.handle.net/11104/0196962