Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification

0358982 - BC 2012 RIV US eng J - Journal Article
Jezbera, Jan - Jezberová, Jitka - Brandt, U. - Hahn, M.W.
Ubiquity of Polynucleobacter necessarius subspecies asymbioticus results from ecological diversification.
Environmental Microbiology. Roč. 13, č. 4 (2011), s. 922-931. ISSN 1462-2912. E-ISSN 1462-2920
R&D Projects: GA ČR(CZ) GAP504/10/0566; GA MŠMT(CZ) MEB060901
Institutional research plan: CEZ:AV0Z60170517
Keywords : ubiquity * Polynucleobacter * ecological diversification
Subject RIV: EE - Microbiology, Virology
Impact factor: 5.843, year: 2011

The subspecies Polynucleobacter necessarius asymbioticus (> 99% 16S rRNA similarity) has a cosmopolitan distribution and a ubiquitous occurrence in freshwater habitats. We tested if the observed ubiquity of these free-living planktonic freshwater bacteria results from a euryoecious (generalist) adaptation of P. n. asymbioticus strains, or from ecological diversification within the subspecies. We developed a reverse line blot hybridization assay enabling the cultivation-independent detection of 13 groups within the subspecies in environmental samples. A set of 121 lentic freshwater habitats, spanning a broad variety of habitat types (e.g. pH levels ranging from 3.8 to 8.5) was investigated for the presence of these 13 P. n. asymbioticus groups. Statistical analyses of the RLBH detections revealed pronounced differences in habitat preferences of several of the groups. Their preferences differed regarding pH, conductivity, dissolved organic carbon and oxygen concentration of habitats.
Permanent Link: http://hdl.handle.net/11104/0196869