Single-step electrospinning to bioactive polymer nanofibers

0358513 - ÚMCH 2012 RIV US eng J - Journal Article
Gentsch, R. - Pippig, F. - Schmidt, S. - Černoch, Peter - Polleux, J. - Börner, H. G.
Single-step electrospinning to bioactive polymer nanofibers.
Macromolecules. Roč. 44, č. 3 (2011), s. 453-461. ISSN 0024-9297. E-ISSN 1520-5835
Institutional research plan: CEZ:AV0Z40500505
Keywords : electrospinning * polymer-peptide conjugate * block copolymer
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 5.167, year: 2011

A single-step process to get polymer nanofiber meshes of biofunctional peptide segments is described in the article. A standard electrospinning of a homogeneous mixture of a polymer-peptide conjugate (poly(lactic acid)-block-CGGRGDS) and a biocompatible commodity poly(lactic-co-glycolic acid) (PLGA) leads to nonwovens with the peptide part enriched on the fiber surface. The accessibility of the peptide is proved by contact angle measurements as well as by probing electrostatic interaction between CGGRGDS surface functionalities and a colloidal silica probe via AFM. Bioavailability and bioactivity tests on the fiber surface demonstrate that the meshes promote adhesion and migration of fibroblasts in comparison to pure PLGA meshes. The one-step production of PLGA-based fibers could be exploited to electrospin into living cell culture without indication of toxic adverse effects on cell proliferation. This might be useful for production of cell-loaded scaffolds or biohybrid materials.
Permanent Link: http://hdl.handle.net/11104/0006401