Monte Carlo simulation of fluorescence correlation spectroscopy data

0358508 - ÚFCH JH 2012 RIV CZ eng J - Journal Article
Košovan, P. - Uhlík, F. - Kuldová, J. - Štěpánek, M. - Limpouchová, Z. - Procházka, K. - Benda, Aleš - Humpolíčková, Jana - Hof, Martin
Monte Carlo simulation of fluorescence correlation spectroscopy data.
Collection of Czechoslovak Chemical Communications. Roč. 76, č. 3 (2011), s. 207-222. ISSN 0010-0765
R&D Projects: GA AV ČR IAA400400621
Institutional research plan: CEZ:AV0Z40400503
Keywords : Monte Carlo Study * fluorescence * spectroscopy
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 1.283, year: 2011

We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones.
Permanent Link: http://hdl.handle.net/11104/0196531