Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois

0358425 - ÚBO 2012 RIV CZ eng J - Journal Article
Zemanová, Barbora - Hájková, Petra - Bryja, Josef - Zima Jr., Jan - Hájková, Andrea - Zima, Jan
Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois.
Folia zoologica. Roč. 60, č. 1 (2011), s. 70-80. ISSN 0139-7893. E-ISSN 1573-1189
R&D Projects: GA AV ČR IAA600930609
Institutional research plan: CEZ:AV0Z60930519
Keywords : noninvasive genetic sampling * amplification success rate * ungulates
Subject RIV: EG - Zoology
Impact factor: 0.554, year: 2011
http://www.ivb.cz/folia/60/1/11_2011.pdf

The only autochthonous population of Tatra chamois (Rupicapra rupicapra tatrica) occurs in the Tatra Mountains (N Slovakia and S Poland). Another population has been introduced to the Low Tatra Mts., while Alpine chamois (R. r. rupicapra) has been introduced to the neighbouring mountain ranges, Veľká Fatra and Slovenský raj. All these populations have undergone intensive bottlenecks, which could result in prevailing monomorphism of available microsatellite markers. We tested 65 markers previously used in chamois or other Caprinae species, from which 20 most suitable loci for noninvasive genetic study of the Tatra chamois were used for optimisation of three multiplex sets. Low genetic diversity was observed in the Tatra chamois populations while slightly higher values were obtained for Alpine chamois population in Slovenský raj. We subsequently assessed the amplification success rate for noninvasively obtained samples (faeces).
Permanent Link: http://hdl.handle.net/11104/0196477