Molecular evidence for the existence of cryptic species assemblages of several myxosporeans (Myxozoa)

0358336 - BC 2012 RIV DE eng J - Journal Article
Bartošová, Pavla - Fiala, Ivan
Molecular evidence for the existence of cryptic species assemblages of several myxosporeans (Myxozoa).
Parasitology Research. Roč. 108, č. 3 (2011), s. 573-583. ISSN 0932-0113. E-ISSN 1432-1955
R&D Projects: GA AV ČR KJB600960701; GA ČR GD524/03/H133; GA MŠMT LC522
Institutional research plan: CEZ:AV0Z60220518
Keywords : SUBUNIT RIBOSOMAL-RNA * RUTILUS-RUTILUS LINNAEUS * LIFE-CYCLE * PHYLOGENY
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.149, year: 2011

Myxosporeans Chloromyxum cristatum, Chloromyxum fluviatile and Zschokkella nova (Myxozoa) are common gall bladder parasites of the cyprinid fishes frequently persisting as co-infections. Despite the fact that they are believed to be innocuous endocommensals, C. cristatum clearly displays the potential of a serious pathogen since it may pervade fish liver parenchyma and cause its necrosis. Employing the comparison of genetic distances among the myxosporean rDNA sequences and performing phylogenetic analyses, we demonstrate that cryptic species assemblages exist in C. fluviatile and Z. nova. Sequence comparison revealed that Chloromyxum legeri, previously assigned as a junior synonym of C. fluviatile, is a valid species. We show that C. cristatum is not an assemblage of more species, and our results support the synonymy of Chloromyxum cyprini with C. cristatum. We have developed a multiplex PCR as an effective tool for the detection and discrimination of the studied pathogens.
Permanent Link: http://hdl.handle.net/11104/0196413