Surface Plasmon Resonance Biosensor for Rapid Label-Free Detection of Microribonucleic Acid at Subfemtomole Level

0358219 - ÚFE 2011 RIV US eng J - Journal Article
Šípová, Hana - Zhang, S.-Ch. - Dudley, A.M. - Galas, D. - Wang, K. - Homola, Jiří
Surface Plasmon Resonance Biosensor for Rapid Label-Free Detection of Microribonucleic Acid at Subfemtomole Level.
Analytical Chemistry. Roč. 82, č. 24 (2010), s. 10110-10115. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA AV ČR KAN200670701
Institutional research plan: CEZ:AV0Z20670512
Keywords : surface plasmon resonance * microRNA * cancer diagnostics * biosensor
Subject RIV: JB - Sensors, Measurment, Regulation
Impact factor: 5.874, year: 2010

MicroRNAs (miRNAs) present an important target for medical diagnostics of various serious diseases. We report a novel method for rapid and sensitive miRNA detection and quantization using high-performance portable surface plasmon resonance (SPR) sensor combined with a DNA*RNA antibody-based assay. The new approach allows detecting miRNA at levels down to high attomoles in less than 30 minutes. The methodology is found to yield results which agree well with established methods for miRNA detection.
Permanent Link: http://hdl.handle.net/11104/0196316