Lignification and related parameters in copper-exposed Matricaria chamomilla roots: Role of H2O2 and NO in this process

0357896 - ÚEB 2011 RIV IE eng J - Journal Article
Kováčik, J. - Grúz, Jiří - Klejdus, B. - Štork, F. - Marchiosi, R. - Ferrarese-Filho, O.
Lignification and related parameters in copper-exposed Matricaria chamomilla roots: Role of H2O2 and NO in this process.
Plant Science. Roč. 179, č. 4 (2010), s. 383-389. ISSN 0168-9452. E-ISSN 1873-2259
Institutional research plan: CEZ:AV0Z50380511
Keywords : Heavy metals * Oxidative stress * Phenols
Subject RIV: EF - Botanics
Impact factor: 2.481, year: 2010

Despite many investigations of the role of ROS and NO in plant metabolism, their involvement in lignification is still poorly understood. We tested the regulatory role of hydrogen peroxide (scavenged by dithiothreitol, DTT) and nitric oxide (scavenged by 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxy1-3-oxide, PTIO) using chamomile roots exposed to Cu for 24 h. The application of DTT and PTIO led to a compensatory increase in NO and H2O2, respectively. DTT prevented Cu-induced lignin accumulation concomitant with a decrease in H2O2 content and the activities of guaiacol- and ascorbate peroxidases. Cinnamyl alcohol dehydrogenase (CAD) and polyphenol oxidase (PPO) also decreased. DTT stimulated accumulation of total soluble phenols and phenolic acids in all three fractions investigated. The opposite trend was recorded in PTIO-treated roots. Within the free amino acids, proline and phenylalanine increased in response to DTT application.
Permanent Link: http://hdl.handle.net/11104/0196072