Polymeric nanocapsules ultra stable in complex biological media

0357473 - ÚMCH 2011 RIV NL eng J - Journal Article
Rodriguez-Emmenegger, Cesar Adolfo - Jäger, Alessandro - Jäger, Eliezer - Štěpánek, Petr - Bologna Alles, A. - Guterres, S. S. - Pohlmann, A. R. - Brynda, Eduard
Polymeric nanocapsules ultra stable in complex biological media.
Colloids and Surfaces B-Biointerfaces. Roč. 83, č. 2 (2011), s. 376-381. ISSN 0927-7765. E-ISSN 1873-4367
R&D Projects: GA AV ČR KAN200670701; GA ČR GAP208/10/1600
Institutional research plan: CEZ:AV0Z40500505
Keywords : core/shell nanocapsules * biodegradable * antifouling coating
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 3.456, year: 2011

Nanocapsules (NC) prepared from FDA-approved degradable poly( -caprolactone) shell and Mygliol 812® oil in the core were coated with oligo(ethylene glycol) methacrylate (poly(MeOEGMA)) polymer brushes with a well-controlled thickness up to 350 nm using surface initiated atom transfer radical polymerization in water or phosphate buffered saline. Incubation of uncoated NC with human serum albumin solution, fetal bovine serum, or human blood plasma resulted in fast aggregation observed by dynamic light scattering as an increase in diameter of particles present in the solutions. Conversely, these biological fluids affected only marginally the size distribution of the NC coated with a 60 nm thick poly(MeOEGMA) layer. The high suspension stability of the coated NC in complex biological fluids was related to the suppressed deposition of proteins from these fluids observed by surface plasmon resonance (SPR) on analogous poly(MeOEGMA) layer prepared on flat surfaces of SPR chips.
Permanent Link: http://hdl.handle.net/11104/0195742