Biochemical properties of three plant nucleases with anticancer potential

0357370 - BC 2012 RIV IE eng J - Journal Article
Podzimek, Tomáš - Matoušek, Jaroslav - Lipovová, P. - Poučková, P. - Spiwok, V. - Šantrůček, J.
Biochemical properties of three plant nucleases with anticancer potential.
Plant Science. Roč. 180, č. 2 (2011), s. 343-351. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA ČR GA521/09/1214
Institutional research plan: CEZ:AV0Z50510513
Keywords : dsRNase activity * N-glycosylation * Anti-tumor effect
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.945, year: 2011

Biochemical and structural properties of three recombinant (R), highly homologous, plant bifunctional nucleases from tomato (R-TBN1), hop (R-HBN1) and Arabis brassica (R-ABN1) were determined. These nucleases cleave single- and double-stranded substrates, as well as both RNA and DNA with nearly the same efficiency. In addition, they are able to cleave several artificial substrates and highly stable viroid RNA. They also possess 3'-nucleotidase activity; therefore, they can be classified as nuclease I family members. Interestingly, poly(G) is resistant to cleavage and moreover it inhibits dsDNase, ssDNase and RNase activity of the studied nucleases. All three nucleases exhibit zinc-dependence and a strong stimulatory effect of Zn2+ for dsDNA cleavage. 3-D models, predicted on the basis of experimental structure of P1 nuclease, show nine amino acid residues responsible for interactions with zinc atoms, located in the same positions as in P1 nuclease.
Permanent Link: http://hdl.handle.net/11104/0195659