Defence responses induced in embryogenic cultures of Norway spruce by two fractions of Gremmeniella abietina mycelia

0357022 - ÚEB 2011 RIV DE eng J - Journal Article
Cvikrová, Milena - Malá, J. - Hrubcová, Marie - Martincová, Olga - Cvrčková, H. - Lipavská, H.
Defence responses induced in embryogenic cultures of Norway spruce by two fractions of Gremmeniella abietina mycelia.
Forest Pathology. Roč. 40, č. 6 (2010), s. 467-484. ISSN 1437-4781. E-ISSN 1439-0329
R&D Projects: GA MZe QH82303
Institutional research plan: CEZ:AV0Z50380511
Keywords : defence response * Norway spruce * Gremmeniella abietina
Subject RIV: GK - Forestry
Impact factor: 0.948, year: 2010

We have examined defence responses in embryogenic cell suspension cultures of Norway spruce elicited by intracellular protein and wall fractions (PF and WF) prepared from mycelia of the fungus Gremmeniella abietina, focusing on changes in levels of phenolics, polyamines and activities of enzymes that catalyse important steps in their metabolism: phenylalanine ammonia-lyase and diamine oxidase. Treatment with both preparations induced increases in the spruce cells total contents of phenolic acids and polyamines but they responded more rapidly to WF than to PF. The contents of soluble and cell wall-bound phenolic acids and soluble conjugates of putrescine started to increase relative to controls (C) within 4 h of the addition of the WF preparation. These changes correlated with the induction of PAL activity in spruce cells at 4 and 8 h following the addition of WF and PF preparations. Increases in DAO activity were also detected after 4 h exposure to WF, and after 24 h exposure to PF.
Permanent Link: http://hdl.handle.net/11104/0195394