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Facile end-labeling of RNA with electroactive Os(VI) complexes
- 1.0356812 - BFÚ 2011 RIV US eng J - Journal Article
Trefulka, Mojmír - Bartošík, Martin - Paleček, Emil
Facile end-labeling of RNA with electroactive Os(VI) complexes.
Electrochemistry Communications. Roč. 12, č. 12 (2010), s. 1760-1763. ISSN 1388-2481. E-ISSN 1873-1902
R&D Projects: GA ČR(CZ) GPP301/10/P548; GA MŠMT(CZ) ME09038; GA MŠMT(CZ) LC06035
Grant - others:GA AV ČR(CZ) KAN400310651
Program: KA
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : RNA end-labeling * voltammetry * osmium complexes
Subject RIV: BO - Biophysics
Impact factor: 4.282, year: 2010
Ribose at the 3'-end of oligonucleotides (oligos) selectively modified by Os(VI)2,2'-bipyridine (bipy) produced two CV redox couples at pyrolytic graphite electrode. Using square wave voltammetry (SWV) 22-mer oligos can be detected down to 250 nM. At mercury electrodes the Os(VI)bipy-oligo adducts produced an electrocatalytic peak at ~-1.2 V allowing their determination down to picomolar concentrations.
Permanent Link: http://hdl.handle.net/11104/0195238
Number of the records: 1