Absorbance changes accompanying the fast fluorescence induction in the purple bacterium Rhodobacter sphaeroides

0356395 - BC 2012 RIV NL eng J - Journal Article
Bína, David - Litvín, Radek - Vácha, František
Absorbance changes accompanying the fast fluorescence induction in the purple bacterium Rhodobacter sphaeroides.
Photosynthesis Research. Roč. 105, č. 2 (2010), s. 115-121. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA AV ČR IAA608170603
Institutional research plan: CEZ:AV0Z50510513
Keywords : Photosynthesis * Charge separation * Membrane potential
Subject RIV: CE - Biochemistry
Impact factor: 2.410, year: 2010

The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in the reaction center and the membrane potential. The results of this study support the view that the membrane potential act predominantly to increase the fluorescence yield. Advantages of the pulsed actinic illumination for study of the operation of the electron transport chain in vivo are discussed.
Permanent Link: http://hdl.handle.net/11104/0194925