Enzymatic Reaction – Fractionation Process under SC-CO2 as a Way to Concentrate DHA from Hake Skin Oil

0356221 - ÚCHP 2011 AT eng C - Conference Paper (international conference)
Rubio-Rodrígueza, N. - Rochová, Kristina - Sovová, Helena - Beltrána, S.
Enzymatic Reaction – Fractionation Process under SC-CO2 as a Way to Concentrate DHA from Hake Skin Oil.
Abstracts. Graz: -, 2010, s. 101. ISBN 978-2905267-72-6.
[European Meeting on Supercritical Fluids /12./. Graz (AT), 09.05.2010-12.05.2010]
Grant - others:MICINN(ES) CTQ2005-07301; JCyL(ES) GR167; S.A.:(ES) A19J.06
Institutional research plan: CEZ:AV0Z40720504
Keywords : omega-3 concentrates * enzymatic reaction * supercritical CO2
Subject RIV: CI - Industrial Chemistry, Chemical Engineering

A continuous enzymatic hydrolysis–fractionation process under SC-CO2 is proposed as a method to concentrate DHA, as acylglyceride, from hake oil. To evaluate the reaction kinetics, hydrolysis experiments were carried out with a sn-1,3 specific lipase from Mucor miehi immobilized on a macroporous ion-exchange resin at 313 K, pressures and CO2 flow rates (0.5-1.9 g/min). Fixing the reaction conditions at 313 K, 25 MPa and 0.5 g CO2/min, a subsequent fractionation in two separators S1 and S2 in series was studied changing pressure (8.3-25 MPa) and temperature (308-333 K) in S1. The best separation factors between acylglyceride and FFA, and DHA and palmitic acid respectively, were obtained working in S1 at a density close to the critical density of CO2. DHA was slightly concentrated as acylglyceride S1.
Permanent Link: http://hdl.handle.net/11104/0194800