A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin

0355821 - ÚMG 2011 RIV DE eng J - Journal Article
Sobol, Margaryta - Nebesářová, Jana - Hozák, Pavel
A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin.
Histochemistry and Cell Biology. Roč. 135, č. 1 (2011), s. 103-110. ISSN 0948-6143. E-ISSN 1432-119X
R&D Projects: GA AV ČR KAN200520704; GA MŠMT 2B06063; GA MŠMT LC545
Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z60220518
Keywords : Mitotic cells * High-pressure freezing * Histochemistry
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.588, year: 2011

The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still missing for mitotic cells especially because of their fragility and sensitivity to treatments. Here, we present a fast and effective method for HPF/automated FS and LR White embedding of mitotic cells which allows for their controlled and reproducible quality processing. It should be useful in various ultrastructural studies on mitotic cells especially in combination with immunocytochemistry.
Permanent Link: http://hdl.handle.net/11104/0194501