Fragmentation of Human Erythrocyte Actin following Exposure to Hypoxia

0355472 - MBÚ 2011 RIV CH eng J - Journal Article
Risso, A. - Santamaria, B. - Pistarino, E. - Cosulich, M. E. - Pompach, Petr - Bezouška, Karel - Antonutto, G.
Fragmentation of Human Erythrocyte Actin following Exposure to Hypoxia.
Acta Haematologica. Roč. 123, č. 1 (2010), s. 6-13. ISSN 0001-5792. E-ISSN 1421-9662
Institutional research plan: CEZ:AV0Z50200510
Keywords : beta-Actin * Erythrocytes * Hypoxia
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.316, year: 2010

In a comparative study on erythrocytes (RBCs) drawn from mountaineers before and after a high-altitude stay, we observed that upon returning to sea level, their RBCs displayed a senescent-like phenotype as indicated by their density and the partial loss of membrane proteins which are shed by ageing RBCs. The aim of this study was to investigate possible changes in the membrane skeleton of these RBCs and to compare them with pathological RBCs. We analysed the proteins of RBC ghosts obtained from our subjects before and after returning to sea level by two-dimensional electrophoresis and mass spectrometry. We observed lower expression and fragmentation of -actin after exposure to hypoxia. This suggested an alteration in membrane skeleton structure, which was confirmed by -actin release in cell lysates during ghost preparation
Permanent Link: http://hdl.handle.net/11104/0194232