Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP

0355190 - ÚMG 2011 RIV GR eng J - Journal Article
Tolde, O. - Rosel, D. - Mierke, C.T. - Paňková, D. - Folk, P. - Veselý, Pavel - Brabek, J.
Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP.
International Journal of Oncology. Roč. 36, č. 4 (2010), s. 833-839. ISSN 1019-6439. E-ISSN 1791-2423
R&D Projects: GA MŠMT(CZ) LC06061
Institutional research plan: CEZ:AV0Z50520514
Keywords : invasiveness * neoplastic progression * cytoskeletal dynamics
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.571, year: 2010

The newly established breast cancer cell line G3S1, derived from EM-G3 breast cancer progenitors. was analyzed for functional changes related to neoplastic progression manifested by elevated invasiveness and enhanced capability to degrade gelatin. Degradation of gelatin and invasiveness of G3S1 cells was found to be dependent on the activity of matrix proteinases and actin cytoskeletal dynamics. Therefore, the expression and activity of these proteases was compared in G3S1 and EM-G3 cells. Despite enhanced capability of G3S1 cells to degrade gelatin. these cells exhibited lower levels of secreted extracellular matrix degrading proteases than parental EM-G3 cells. However, the expression of membrane-bound MT1-MMP was strongly elevated in G3S1 cells. While the degradation of gelatin was associated with invadopodia-like structures in both EM-G3 and G3S1 cells, the cytoskeletal remodeling dynamics was greatly elevated in G3S1 cells.
Permanent Link: http://hdl.handle.net/11104/0194017