Restoration of spermatogenesis after transplantation of c-Kit positive testicular cells in the fowl

0355186 - ÚMG 2011 RIV US eng J - Journal Article
Trefil, P. - Bakst, M.R. - Yan, H. - Hejnar, Jiří - Kalina, J. - Mucksová, J.
Restoration of spermatogenesis after transplantation of c-Kit positive testicular cells in the fowl.
Theriogenelogy. Roč. 74, č. 9 (2010), s. 1670-1676. ISSN 0093-691X. E-ISSN 1879-3231
R&D Projects: GA ČR GA523/07/1171
Institutional research plan: CEZ:AV0Z50520514
Keywords : chicken * transplantation * c-Kit receptor
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.045, year: 2010

Transplantation of male germ line cells Into sterilized recipients has been used in mammals for conventional breeding as well as for transgenesis We have previously adapted this approach for the domestic chicken and we present now an improvement of the germ cell transplantation technique by using an enriched subpopulation of c-Kit positive spermatogonia as donor cells Dispersed c-Kit positive testicular cells from 16 to 17 week-old pubertal donors were transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation We describe the repopulation of the recipient s testes with c Kit positive donor testicular cells, which resulted in the production of functional heterologous spermatozoa
Permanent Link: http://hdl.handle.net/11104/0194015