Spatiotemporal Dynamics of Glycolytic Waves Provides New Insights into the Interactions Between Immobilized Yeast Cells and Gels

0355106 - ÚCHP 2011 RIV NL eng J - Journal Article
Bolyó, Juraj - Mair, T. - Kuncová, Gabriela - Hauser, M.J.B.
Spatiotemporal Dynamics of Glycolytic Waves Provides New Insights into the Interactions Between Immobilized Yeast Cells and Gels.
Biophysical Chemistry. Roč. 153, č. 1 (2010), s. 54-60. ISSN 0301-4622. E-ISSN 1873-4200
R&D Projects: GA MŠMT OC 121
Grant - others:DF(DE) MU5939/19-1; ESF(XE) COST 865
Institutional research plan: CEZ:AV0Z40720504
Keywords : eaction–diffusion-coupling * pattern formation * metabolic synchronization
Subject RIV: CE - Biochemistry
Impact factor: 2.108, year: 2010

We show that the spatiotemporal dynamics of waves of metabolic synchronization of yeast cells provides a new criterion to distinguish the effect of different gels on the cellular metabolism, which otherwise could not be detected. Cells from the yeast Saccharomyces carlsbergensis were immobilized into agarose gel, silica gel (TMOS), or a mixture of TMOS and alginate. We compared these immobilized cells with respect to their ability to generate temporal, intracellular oscillations in glycolysis as well as propagating, extracellular synchronization waves. While the temporal dynamics, as measured by the period and the number of oscillatory cycles, was similar for all three immobilized cell populations, significant differences have been observed with respect to the shape of the waves, wave propagation direction and velocity in the three gel matrices used.
Permanent Link: http://hdl.handle.net/11104/0193949