Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes

0355014 - ÚMG 2011 RIV GB eng J - Journal Article
Sarnová, Lenka - Malík, Radek - Sedláček, Radislav - Svoboda, Petr
Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.
Journal of negative results in biomedicine. Roč. 9, č. 8 (2010), s. 1-10. ISSN 1477-5751
R&D Projects: GA MŠMT ME09039
Grant - others:EMBO SDIG(DE) project 1483
Institutional research plan: CEZ:AV0Z50520514
Keywords : transgenic RNAi * shRNA * oocyte
Subject RIV: EB - Genetics ; Molecular Biology
http://www.jnrbm.com/content/9/1/8

So far, transgenic RNAi in mouse oocytes has been using long dsRNA (over 500 bp long). Although it worked well in the oocyte, this technology cannot be used in somatic cells and it requires a considerable amount of work. Therefore, we tested a universal shRNA system placed in a retrovirus-compatible vector. Our results show that the shRNA technology does not bring any advantage in the amount of labor, time and cost and, furthermore, this particular type of vector is efficiently epigenetically silenced in transgenic animals.
Permanent Link: http://hdl.handle.net/11104/0193876