Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis

0354589 - MBÚ 2011 RIV CZ eng J - Journal Article
Petráčková, Denisa - Šemberová, L. - Halada, Petr - Svoboda, Petr - Svobodová, J.
Stress Proteins in the Cytoplasmic Membrane Fraction of Bacillus subtilis.
Folia Microbiologica. Roč. 55, č. 5 (2010), s. 427-434. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA MŠMT LC07017
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z50110509
Keywords : FUNCTION SIGMA-FACTOR * SACCHAROMYCES-CEREVISIAE * IDENTIFICATION
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.977, year: 2010

Stress proteomes of the cytoplasmic membrane fraction of Bacillus subtilis trp (C2)-exposed to acid pH and ethanol were characterized. Although these stress factors impair the cell function in a specific manner, they share the ability to denature proteins. Therefore, specific and general stress proteins in the membranes were investigated. Both ethanol (3 percent) and pH 5.0 increase the doubling time from 17 to 25 min. Isolated cytoplasmic membranes were subjected to an optimized 2D PAGE analysis which permitted the separation and analysis of 450 distinct protein spots. Two alternative methods of protein detection were compared, i.e. silver staining and (35)S-L-methionine pulse labeling; the stress induced proteins were identified by MALDI-TOF MS. After ethanol stress, five proteins were increased, viz. YdaP, Ctc, YfhM, YjcH and YwaC. Acid stress proteins were AcoB, YkwC, SodA, YjcH and YwaC. Proteins YjcH and YwaC were increased after ethanol as well as acid pH treatment
Permanent Link: http://hdl.handle.net/11104/0193559