Comparative Study of the Life Cycle Dependent Post-Translation Modifications of Protein Synthesis Elongation Factor Tu Present in the Membrane Proteome of Streptomycetes and Mycobacteria

0354457 - MBÚ 2011 RIV CZ eng J - Journal Article
Holub, Martin - Bezoušková, Silvia - Petráčková, Denisa - Kalachová, Ladislava - Kofroňová, Olga - Benada, Oldřich - Weiser, Jaroslav
Comparative Study of the Life Cycle Dependent Post-Translation Modifications of Protein Synthesis Elongation Factor Tu Present in the Membrane Proteome of Streptomycetes and Mycobacteria.
Folia Microbiologica. Roč. 55, č. 3 (2010), s. 203-210. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA AV ČR IAA600200702; GA AV ČR IAA500200913
Institutional research plan: CEZ:AV0Z50200510
Keywords : ESCHERICHIA-COLI * COELICOLOR A3(2) * OUTER-MEMBRANE
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.977, year: 2010

We present the results of analysis of membrane phosphoproteomes from individual morphological stages of Streptomyces coelicolor that reflect developmentally dependent heterogeneity and phosphorylation of intrinsic and externally added purified Strepomyces aureofaciens EF-Tu. Fast growing nonpathogenic Mycobacterium smegmatis was used as a non-differentiating actinomycetes comparative model. Streptomycetes membrane fraction was found to contain protein kinase(s) catalyzing phosphorylation of both its own and an externally added EF-Tu, whereas Mycobacterium membrane fraction contains protein kinase phosphorylating only its own EF-Tu
Permanent Link: http://hdl.handle.net/11104/0193454