Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology

0354405 - FGÚ 2011 RIV US eng J - Journal Article
Burdíková, Zuzana - Čapek, Martin - Ostašov, Pavel - Machač, Jiří - Pelc, Radek - Mitchell, E.A.D. - Kubínová, Lucie
Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology.
Microscopy and Microanalysis. Roč. 16, č. 6 (2010), s. 735-746. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA MŠMT(CZ) LC06063; GA ČR(CZ) GA102/08/0691
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z60050516
Keywords : testate amoebae * confocal microscopy * environmental scanning electron microscopy
Subject RIV: JD - Computer Applications, Robotics
Impact factor: 2.179, year: 2010

In our study we applied both traditional and advanced microscopic imaging techniques to study testate amoebae (TA) shell and cell morphology. When compared with scanning electron microscopy (SEM) or environmental SEM (ESEM), confocal laser scanning microscopy (CLSM) and two-photon excitation microscopy (TPEM) offered several additional advantages for investigations requiring information of morphological characteristics, useful for taxonomical and ecophysiological research: (1) CLSM and TPEM allow imaging of inner structures of TA in contrast to SEM or ESEM. (2) CLSM and TPEM can simplify and refine studies of morphology. (3) CLSM and TPEM allow us to obtain data that can be used for stereological estimations of volumes of TA. (4) Fluorescence markers allow us to see structures not visible in transmitting light
Permanent Link: http://hdl.handle.net/11104/0193414