The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning

0354389 - MBÚ 2011 RIV US eng J - Journal Article
Cuchalová, Lucie - Kouba, Tomáš - Herrmannová, Anna - Dányi, István - Chiu, W.-L. - Valášek, Leoš
The RNA Recognition Motif of Eukaryotic Translation Initiation Factor 3g (eIF3g) Is Required for Resumption of Scanning of Posttermination Ribosomes for Reinitiation on GCN4 and Together with eIF3i Stimulates Linear Scanning.
Molecular and Cellular Biology. Roč. 30, č. 19 (2010), s. 4671-4686. ISSN 0270-7306. E-ISSN 1098-5549
Institutional research plan: CEZ:AV0Z50200510
Keywords : OPEN READING FRAMES * START CODON SELECTION * MESSENGER-RNA
Subject RIV: EE - Microbiology, Virology
Impact factor: 6.188, year: 2010

Recent reports have begun unraveling the details of various roles of individual eukaryotic translation initiation factor 3 (eIF3) subunits in translation initiation. Here we describe functional characterization of two essential Saccharomyces cerevisiae eIF3 subunits, g/Tif35 and i/Tif34, previously suggested to be dispensable for formation of the 48S preinitiation complexes (PICs) in vitro. A triple-Ala substitution of conserved residues in the RRM of g/Tif35 (g/tif35-KLF) or a single-point mutation in the WD40 repeat 6 of i/Tif34 (i/tif34-Q258R) produces severe growth defects and decreases the rate of translation initiation in vivo without affecting the integrity of eIF3 and formation of the 43S PICs in vivo. Both mutations also diminish induction of GCN4 expression, which occurs upon starvation via reinitiation
Permanent Link: http://hdl.handle.net/11104/0193400