Changes in Cell Adhesivity and Cytoskeleton-Related Proteins During Imatinib-Induced Apoptosis of Leukemic JURL-MK1 Cells

0354386 - MBÚ 2011 RIV US eng J - Journal Article
Kuželová, K. - Pluskalová, M. - Grebeňová, D. - Pavlásková, Kateřina - Halada, Petr - Hrkal, Z.
Changes in Cell Adhesivity and Cytoskeleton-Related Proteins During Imatinib-Induced Apoptosis of Leukemic JURL-MK1 Cells.
Journal of Cellular Biochemistry. Roč. 111, č. 6 (2010), s. 1413-1425. ISSN 0730-2312. E-ISSN 1097-4644
R&D Projects: GA MŠMT LC07017; GA MZd NR9243
Institutional research plan: CEZ:AV0Z50200510
Keywords : imatinib * adhesion * cytoskeleton
Subject RIV: CE - Biochemistry
Impact factor: 3.122, year: 2010

The fusion protein Bcr–Abl, which is the molecular cause of chronic myelogenous leukemia (CML) interacts in multiple points with signaling pathways regulating the cellular adhesivity and cytoskeleton architecture and dynamics. We explored the effects of imatinib mesylate, an inhibitor of Bcr–Abl protein used in front-line CML therapy, on the adhesivity of JURL-MK1 cells to fibronectin and searched for underlying changes in the cell proteome. As imatinib induces apoptosis of JURL-MK1 cells, we used three different caspase inhibitors to discriminate between direct consequences of Bcr–Abl inhibition and secondary changes related to the apoptosis. Imatinib treatment caused a transient increase in JURL-MK1 cell adhesivity to fibronectin, possibly due to the switch off of Bcr–Abl activity
Permanent Link: http://hdl.handle.net/11104/0193397