Integrated liquid chromatography–heated nebulizer microchip for mass spectrometry

0354131 - MBÚ 2011 RIV NL eng J - Journal Article
Haapala, M. - Saarela, V. - Pól, Jaroslav - Kolari, K. - Kotiaho, T. - Franssila, S. - Kostiainen, R.
Integrated liquid chromatography–heated nebulizer microchip for mass spectrometry.
Analytica Chimica Acta. Roč. 662, č. 2 (2010), s. 163-169. ISSN 0003-2670. E-ISSN 1873-4324
Institutional research plan: CEZ:AV0Z50200510
Keywords : Liquid chromatography * Mass spectrometry * Microchip
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.310, year: 2010

A new integrated microchip for liquid chromatography–mass spectrometry (LC–MS) is presented. The chip is made from bonded silicon and glass wafers with structures for a packed LC column channel, a micropillar frit, a channel for optional optical detection, and a heated vaporizer section etched in silicon and platinum heater elements on the glass cover. LC eluent is vaporized and mixed with nebulizer gas in the vaporizer section and the vapor is sprayed out from the chip. Nonpolar and polar analytes can be efficiently ionized in the gas phase by atmospheric pressure photoionization (APPI) as demonstrated with polycyclic aromatic hydrocarbons (PAHs) and selective androgen receptor modulators (SARMs). This is not achievable with present LC–MS chips, since they are based on electrospray ionization, which is not able to ionize nonpolar compounds efficiently
Permanent Link: http://hdl.handle.net/11104/0193201