Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae

0354019 - FGÚ 2011 RIV US eng J - Journal Article
Burdíková, Zuzana - Čapek, Martin - Ostašov, Pavel - Mitchell, E.A.D. - Machač, Jiří - Kubínová, Lucie
Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae.
Microscopy and Microanalysis. Roč. 16, Suppl.2 (2010), s. 1142-1143. ISSN 1431-9276. E-ISSN 1435-8115.
[Microscopy and Microanalysis 2010. Portland, 01.08.2010-05.08.2010]
R&D Projects: GA MŠMT(CZ) LC06063; GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z60050516
Keywords : testate amoebae * confocal microscopy * two-photon microscopy
Subject RIV: BH - Optics, Masers, Lasers
Impact factor: 2.179, year: 2010

We applied CLSM and Two-Photon Excitation (TPE) microscopy techniques to visualize testate amoebae (TA), their test, morphology and physiology. TA are usually examined using SEM and E-SEM. It is not possible to acquire images of living TA and physiology of the amoeba inside the test by SEM or E-SEM. The goal of the study was to examine the potential of CLSM and TPE for imaging of TA. We tried 17 fluorescent dyes to label different structures of TA. CLSM enabled us to acquire images of TA from depths up to 40 μm, whereas TPE was able to penetrate to 60 μm. Stereological methods were employed to estimate the volume of the biomass of TA visualized by CLSM
Permanent Link: http://hdl.handle.net/11104/0193112