High-throughput analysis of tetracycline antibiotics and their epimers in liquid

0353983 - MBÚ 2011 RIV GB eng J - Journal Article
Tylová, Tereza - Olšovská, Jana - Novák, Petr - Flieger, Miroslav
High-throughput analysis of tetracycline antibiotics and their epimers in liquid.
Chemosphere. Roč. 78, č. 4 (2010), s. 353-359. ISSN 0045-6535. E-ISSN 1879-1298
R&D Projects: GA MŠMT 1M06011
Institutional research plan: CEZ:AV0Z50200510
Keywords : Liquid-liquid extraction * Ultra Performance Liquid Chromatography * UV detection
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.155, year: 2010

Antibiotics contained in animal manure can contaminate soil, groundwater and eventually surface and drinking water. To reduce the usage of antibiotics in livestock industry the EU banned their application as growth promoters in 2006. Even though the antibiotics are still used for this purpose and therefore it is necessary to control their applications. An Ultra Performance Liquid Chromatography method (UPLC) with UV detection for determination of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), and doxycycline (DOX) including their epimers in the liquid hog manure was developed. The antibiotics were extracted with ethyl acetate and separated on UPLC BEH Shield RP18 column
Permanent Link: http://hdl.handle.net/11104/0193082