Current Protocols in Chemical Biology

0353703 - ÚOCHB 2011 RIV US eng M - Monography Chapter
Macíčková-Cahová, Hana - Vrábel, Milan - Hocek, Michal
Cross-coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA.
Current Protocols in Chemical Biology. Vol. 2. New York: Wiley Interscience, 2010, s. 1-14. ISBN 9780470559277
Institutional research plan: CEZ:AV0Z40550506
Keywords : nucleoside triphosphates * DNA polymerase * cross-coupling * DNA conjugates
Subject RIV: CC - Organic Chemistry

A novel, efficient, two-step methodology is presented for construction of base-modified oligonucleotides or DNA involving aqueous cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) with terminal acetylenes or arylboronic acids, followed by polymerase incorporation of the modified dNTPs either using primer extension (PEX) or polymerase chain reaction (PCR).
Permanent Link: http://hdl.handle.net/11104/0192869