Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans

0353453 - BC 2011 RIV US eng J - Journal Article
Wicht, B. - Yanagida, T. - Scholz, Tomáš - Ito, A. - Jiménez, J. A. - Brabec, Jan
Multiplex PCR for Differential Identification of Broad Tapeworms (Cestoda: Diphyllobothrium) Infecting Humans.
Journal of Clinical Microbiology. Roč. 48, č. 9 (2010), s. 3111-3116. ISSN 0095-1137. E-ISSN 1098-660X
R&D Projects: GA MŠMT LC522
Institutional research plan: CEZ:AV0Z60220518
Keywords : MOLECULAR EVIDENCE * PACIFIC SALMON * NIHONKAIENSE * Diphyllobothrium * multiplex PCR * the cytochrome c oxidase subunit 1 * mitochondrial DNA
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 4.220, year: 2010

The specific identification of broad tapeworms (genus Diphyllobothrium) infecting humans is very difficult to perform by morphological observation. Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing are too complicated, long, and expensive for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common Diphyllobothrium species infecting humans (D. latum, D. dendriticum, D. nihonkaiense, and D. pacificum), based on a multiplex PCR with the cytochrome c oxidase subunit 1 gene of mitochondrial DNA.
Permanent Link: http://hdl.handle.net/11104/0192700