The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho

0353421 - ÚMG 2011 RIV DE eng J - Journal Article
Tůmová, Magda - Koffer, Anna - Šimíček, Michal - Dráberová, Lubica - Dráber, Petr
The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho.
European Journal of Immunology. Roč. 40, č. 11 (2010), s. 3235-3245. ISSN 0014-2980. E-ISSN 1521-4141
R&D Projects: GA MŠMT 1M0506; GA MŠMT LC545; GA ČR(CZ) GD204/05/H023; GA ČR GA301/09/1826; GA ČR GAP302/10/1759; GA AV ČR KAN200520701
Institutional research plan: CEZ:AV0Z50520514
Keywords : cell activation * cytoskeleton * mast cells
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.942, year: 2010

The transmembrane adaptor protein NTAL participates in signalosome assembly in hematopoietic cells, but its exact role in cell physiology remains enigmatic. This study shows that mast cells from NTAL-deficient mice, responding to Ag alone or in combination with SCF, exhibit reduced spreading on fibronectin, enhanced filamentous actin depolymerization and enhanced migration towards Ag relative to WT cells. No such differences between WT and NTAL(-/-) cells were observed when SCF alone was used as activator. Next the activities of two small GTPases, Rac and Rho, which are important regulators of actin polymerization were examined. Stimulation with Ag and/or SCF enhanced activity of Rac (1,2,3) in both NTAL(-/-) and WT cells. In contrast, RhoA activity decreased and this trend was much faster and more extensive in NTAL(-/-) cells, indicating a positive regulatory role of NTAL in the recovery of RhoA activity.
Permanent Link: http://hdl.handle.net/11104/0192674