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Protein surface shielding agents in protein crystallization
- 1.0353319 - ÚMCH 2011 RIV DK eng J - Journal Article
Hašek, Jindřich
Protein surface shielding agents in protein crystallization.
Journal of Synchrotron Radiation. Roč. 18, Part 1 (2011), s. 50-52. ISSN 0909-0495. E-ISSN 1600-5775.
[International Symposium on Diffraction Structural Biology /3./. Paris - Orsay, 25.05.2010-28.05.2010]
R&D Projects: GA AV ČR IAA500500701; GA ČR GA305/07/1073
Institutional research plan: CEZ:AV0Z40500505
Keywords : protein adhesion * protein surface shielding agents * intermolecular interactions
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 2.726, year: 2011
The molecules adhering temporarily on the surface of protein molecules change the propensity of protein molecules to deposit on the crystal surface in a definite position and orientation. The concepts of competitive adhesion modes and protein surface shielding agents acting on the surface of molecules in a non-equilibrium process of protein crystallization provide a useful platform for the control of crystallization. The desirable goal, i.e. a transient preference of a single dominating adhesion mode between protein molecules during crystallization, leads to uniform deposition of proteins in a crystal. This condition is the most important factor for diffraction quality and thus also for the accuracy of protein structure determination. The presented hypothesis is a generalization of the experimentally well proven behaviour of hydrophilic polymers on the surface of protein molecules of other compounds.
Permanent Link: http://hdl.handle.net/11104/0192598
Number of the records: 1