Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å

0353303 - ÚMCH 2011 RIV GB eng J - Journal Article
Skálová, Tereza - Dušková, Jarmila - Hašek, Jindřich - Štěpánková, Andrea - Kovaľ, Tomáš - Ostergaard, L. H. - Dohnálek, Jan
Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 Å.
Acta Crystallographica Section F-Structural Biology and Crystallization Communications. Roč. 67, č. 1 (2011), s. 27-32. ISSN 1744-3091. E-ISSN 2053-230X
R&D Projects: GA ČR GA305/07/1073; GA AV ČR IAA500500701
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z10100521
Keywords : laccase * potassium hexacyanoferrate * X-ray diffraction
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 0.506, year: 2011

The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date.
Permanent Link: http://hdl.handle.net/11104/0192586