Effect of long-term preservation of basidiomycetes on perlite in liquid nitrogen on their growth, morphological, enzymatic and genetic Characteristics

0352906 - MBÚ 2011 RIV GB eng J - Journal Article
Homolka, Ladislav - Lisá, Ludmila - Eichlerová, Ivana - Valášková, Vendula - Baldrian, Petr
Effect of long-term preservation of basidiomycetes on perlite in liquid nitrogen on their growth, morphological, enzymatic and genetic Characteristics.
Fungal Biology. Roč. 114, 11-12 (2010), s. 929-935. ISSN 1878-6146. E-ISSN 1878-6162
R&D Projects: GA MŠMT LC06066
Institutional research plan: CEZ:AV0Z50200510
Keywords : Basidiomycetes * Cryopreservation * Enzymes
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.000, year: 2010

The macro- and micro-morphological features, mycelial extension rate, enzymatic activities and possible genetic changes were studied in 30 selected strains of basidiomycetes after 10-year cryopreservation on perlite in liquid nitrogen (LN). Comparisons with the same strains preserved by serial transfers on nutrient media at 4 C were also conducted. Production of ligninolytic enzymes and hydrogen peroxide was studied by quantitative spectrophotometric methods, whereas semiquantitative API ZYM testing was used to compare the levels of a wide range of hydrolytic enzymes
Permanent Link: http://hdl.handle.net/11104/0192294