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Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation
- 1.0352888 - MBÚ 2011 RIV NL eng J - Journal Article
Hovorka, Ondřej - Šubr, Vladimír - Větvička, David - Kovář, Lubomír - Strohalm, Jiří - Strohalm, Martin - Benda, Aleš - Hof, Martin - Ulbrich, Karel - Říhová, Blanka
Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation.
European Journal of Pharmaceutics and Biopharmaceutics. Roč. 76, č. 3 (2010), s. 514-524. ISSN 0939-6411. E-ISSN 1873-3441
R&D Projects: GA AV ČR IAA400200702; GA AV ČR IAAX00500803; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z40400503; CEZ:AV0Z40500505
Keywords : doxorubicin * spectral analysis * fluorescence
Subject RIV: EC - Immunology
Impact factor: 4.304, year: 2010
We define two microscopy techniques enabling to distinguish and separate Dox and D* emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst 33342 rather than a direct measurement od doxorubicin fluorescence inside the cells
Permanent Link: http://hdl.handle.net/11104/0192282
Number of the records: 1