Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts usány chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry

0352779 - MBÚ 2011 RIV NL eng J - Journal Article
Myjavcová, R. - Marhol, Petr - Křen, Vladimír - Šimánek, V. - Ulrichová, J. - Palíková, I. - Papoušková, B. - Lemr, Karel - Bednář, P.
Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts usány chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry.
Journal of Chromatography A. Roč. 1217, č. 51 (2010), s. 7932-7941. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MPO FT-TA3/024
Institutional research plan: CEZ:AV0Z50200510
Keywords : anthocyanin * blueberry honeysuckle * chromatography
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.194, year: 2010

Anthocyanins from the fruit Lonicera caerulea L. var. kamtschatica (blueberry honeysuckle, Caprifoliaceae) were studied via (semi)preparative chromatographic fractionation followed by MS and LC/MS analysis. The extraction procedure was optimized with respect to analytical purposes as well as its potential use for the preparation of nutraceuticals. The highest yield of anthocyanins was obtained using acidified methanol as the extraction medium. A comparable total anthocyanin content was obtained using a mixture of methanol and acetone. However, when Lonicera anthocyanins were in contact with acetone, a condensation reaction occurred to a large extent and related 5-methylpyranoanthocyanins were found. The effect of other extraction media, including ethanol as a “green” solvent, is also discussed
Permanent Link: http://hdl.handle.net/11104/0192206