Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth

0352642 - MBÚ 2011 RIV NL eng J - Journal Article
Kameník, Zdeněk - Hadacek, F. - Marečková, M. - Ulanová, Dana - Kopecký, J. - Chobot, V. - Plháčková, Kamila - Olšovská, Jana
Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth.
Journal of Chromatography A. Roč. 1217, č. 51 (2010), s. 8016-8025. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA MŠMT 2B08064
Institutional research plan: CEZ:AV0Z50200510
Keywords : UHPLC * high-throughput fingerprinting * secondary metabolites
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.194, year: 2010

A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector). Thirteen antibiotic standards and four cultivation broths were used for the method development. The comparison of ten liquid–liquid and solid phase extraction protocols for sample clean-up and pre-concentration revealed that Oasis HLB C18 sorbent gives the best recoveries. The Acquity BEH C18 chromatographic column was chosen for the samples separation with respect to its universality, selectivity, efficiency and robustness. The method is presented by two 3D fingerprints for every sample that was obtained under different, acidic and alkaline, UHPLC conditions
Permanent Link: http://hdl.handle.net/11104/0192110